Fig. 5

CREBZF is a directly target gene of circPAPD4/miR-1269a axis in BC cells. (A) Venn diagram of potential target genes of miR-1269a in Starbase, miRDB and microT-CDS. (B) mRNA expression levels of potential target genes (MSL2, SPTLC2, ARAP2, CREBZF) in MCF-7 cells transfected with miR-1269a inhibitors (miR-1269a inh) or miR-NC were detected by RT-qPCR. (C) The mRNA expression of SPTLC2, ARAP2, and CREBZF in MCF-7 cells transfected with OE-circPAPD4 or EV was detected by RT-qPCR. (D) Schematic diagram demonstrated luciferase reporter vectors were inserted with wild-type (WT) or mutant (Mut) potential binding sequence between miR-1269a and CREBZF which was predicted by Starbase. (E) Luciferase reporter vectors loaded with CREBZF wild-type (WT) or mutant (Mut) were co-transfected with miR-1269a mimics (miR-1269a mim) or mimics control (miR-NC), and then relative luciferase activity were assessed to verified the binding relationship between CREBZF and miR-1269a. (F) Pearson’s correlation analysis were used to analysis the correlation among CREBZF, miR-1269a, and circPAPD4 in 50 BC tissues. (G-H) The proliferation and apoptosis of MCF-7 and SKBR3 cells were measured by EdU and TUNEL assays after co-transfected with EV, OE-circPAPD4, OE-circPAPD4 + sh-NC, or OE-circPAPD4 + sh-CREBZF-1. (I) The levels of cyclin-D1, cyclin-E1, BCL-2, and BAX were evaluated by western blotting in MCF-7 and SKBR3 cells as indicated treatments. Data are presented as means ± SD. *p < 0.05; **p < 0.01; ***p < 0.001.