Fig. 7
CREBZF restoration by CREBZF mRNA NPs (CREBZF-mRNA-NPs) exerts anti-tumor effect in vitro. (A) Schematic diagram illustrated that CREBZF-mRNA-NPs consisted of PLGA polymers, lipid-PEG, cationic G0-C14 and CREBZF mRNA. (B) Representative TEM image of CREBZF-mRNA-NPs. Scale bar: 100 nm. (C-D) The hydrodynamic size and zeta potential of CREBZF-mRNA-NPs was evaluated by dynamic light scattering (DLS). (E) Stability of the CREBZF-mRNA-NPs in 10% serum at 37 °C was measure by NanoSIGHT and NTA. (F-G) The restoration of CREBZF by CREBZF-mRNA-NPs was measured by western blotting (F) and immunofluorescence (G). Scale bar: 10 μm. (H-I) The proliferation and apoptosis of CREBZF-null MCF-7 cells as indicated treatments were measured by EDU and TUNEL assays. (J) The half-life period of free Cy5-Luc-mRNA and Cy5-Luc-mRNA NPs in mice blood after i.v. administration. (K) Quantification of biodistribution of free Cy5-Luc-mRNA and Cy5-Luc-mRNA NPs in injection of CREBZF-null MCF-7 cells xenograft models of nude mice. Data are presented as means ± SD. *p < 0.05; **p < 0.01; ***p < 0.001.