Fig. 3

B4GALT1 regulates LUAD cell migration and invasion. A Wound healing assays were used to analyse the migratory activity of A549 and PC9 cells transfected with si-B4GALT1 or pcDNA-B4GALT1. B Transwell assays were used to detect the migration ability of transfected A549 and PC9 cells. C Transwell chambers coated with Matrigel were used to assess invasion after transfection with si-B4GALT1 or pcDNA-B4GALT1 compared with the control groups in A549 and PC9 cells. D Tumours were established in BALB/c nude mice by subcutaneous injection of si-B4GALT1-transfected or control A549 cells. Tumour volumes were calculated after injection every 2 days. The tumour growth curves are shown in the left panel. Tumour weight was assessed from excised tumours (right panel). E Representative images of tumours subjected to H&E staining and IHC staining (B4GALT1, Ki-67). Scale bars, 100 µm. *P < 0.05, **P < 0.01