Fig. 4

B4GALT1 limited CD8 + T-cell infiltration and activity. A Differences in immune composition between the MIA and IAC groups were determined using CIBERSORT. B Representative immunofluorescence staining images of CD3 and CD8 in MIA and IAC tumours. Scale bars, 50 µm. Quantifications are on the right. C Representative immunohistochemical images (left panel) and comparison of H-scores of GZMB in MIA and IAC tumours (right panel). Scale bars, 200 μm. D Representative immunohistochemical images (left panel) and comparison of H-scores of B4GALT1 in MIA and IAC tumours (right panel). Scale bars, 200 μm. E The linear correlations between the H-scores of B4GALT1 and the percentages of CD3, CD8, and H-scores of GAMB in eight tumours from four MPLC. F Log2-fold changes of B4GALT1 expression in the public T-cell coculture screen data. G Illustration showing the in vitro procedure of human and mouse CD8 + T-cell isolation and cocultivation with cancer cells. H LUAD cells cocultured with activated CD8 + T cells for 48 h were subjected to crystal violet staining and quantified at 570 nm. The ratio of LUAD cells to T cells was 1:3. **P < 0.01, n.s., not significant