Fig. 3
From: Ferroptosis inducers enhanced cuproptosis induced by copper ionophores in primary liver cancer
Ferroptosis inducers promoted cuproptosis through depleting intracellular GSH A and B. Relative GSH level in MHCC-97 H (A) or QBC939 (B) cells after treatment with DMSO, 10µM erastin (Era), 10µM sorafenib (Sora), 10nM elesclomol (ES), 10µM Era + 10nM ES or 10µM Sora + 10nM ES. C and D. Cell viability of MHCC-97 H (C) or QBC939 (D) cells after ES 48 h treatment with DMSO, 10µM Sora, 10mM GSH or 10µM Sora + 10mM GSH was measured with CCK-8 assay E. MHCC-97 H cells were treated with indicated drugs for 24 h (DMSO, 10nM ES, 10µM Era + 10nM ES, 10µM Sora + 10nM ES, 10µM Era + 10nM ES + 10mM GSH, 10µM Sora + 10nM ES + 10mM GSH), DLAT protein aggregation was analyzed by immunofluorescence (green, DLAT; red, Mitotracker; blue, DAPI). White scale bars on full tiled images are 5μm F and G. The effect of GSH (10mM) on elesclomol (ES 10nM) and Sora (10µM) or Era (10µM)-induced DLAT protein aggregation was analyzed by western blotting in MHCC-97 H cells. For A to G, media were supplemented with 1µM CuCl2