Fig. 2

CircZFR is an oncogenic circRNA and promotes OXPHOS in vitro. a Heatmap of genes affected by circZFR identified using RNA-seq. b GSEA results used to identify the differential gene profiles. c-e Oxygen consumption rate (OCR) upon cells (c, d) and cellular ATP levels (e) were measured after transfecting with control or circZFR siRNA in A549 and HCC827. f-j CircZFR facilitated the proliferation of A549 cells shown by the RTCA (f, g), EdU (h), cell cycle (i) and apoptosis (j) assays. Cell numbers were determined using the ImageJ program. k Effect of oligomycin (200 nM) on A549 cell vitality detected by RTCA. l Cell viability of A549 cells treated as in (l). Data are shown as mean ± SD (n = 3) or typical photographs of one representative experiment. Similar results were obtained in three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ns, nonsignificant, two-tailed Student’s t test