Fig. 5

MYO1B-fl partially recapitulates the biological function of circZFR via AKT-mTOR signaling. a Subcellular distribution of different MYO1B isoforms. Nuclei was stained with DAPI (blue). Scale bars: 50 μm. b, c The changes of OCR levels (b) and cell vitality (c) in A549 cells after transfecting with control or MYO1B-fl siRNAs. d Western blot of the indicated proteins in the extracts of A549 cells. e The protein levels of MYO1B-fl, phospho-AKT and phospho-mTOR induced by circZFR in A549 cells. f-jMYO1B exons (f), OCR levels (g), cellular ATP (h), cell proliferation (i) and protein expression (j) in A549 cells transfected with HNRNPLL vector alone or co-transfected with MYO1B siRNA. k-oMYO1B exons (k), OCR levels (l), cellular ATP (m), cell proliferation (n) and protein expression (o) in A549 cells transfected with circZFR vector alone or co-transfected with MYO1B siRNA. Data are shown as mean ± SD (n = 3). Similar results were obtained in three independent experiments or typical photographs of one representative experiment. *p < 0.05, **p < 0.01, ***p < 0.001, ANOVA followed by Tukey’s test