Fig. 2

Schemes showing the HOTAIR functions in transcriptional regulation of gene expression. HOTAIR scaffolds distinct repressive histone modification activities (i.e. H3K27 trimethylation by EZH2 component of PRC2 complex, H3K4 and H3K9 demethylation and deacetylation by LSD1/CoREST/REST repressor complex). The targeting of this molecular platform to specific loci of the genome, resulting in the EMT induction, can be dependent (A) on the direct formation of triplex helices between the lncRNA and the double stranded DNA target or (B) on the formation of tripartite complexes with specific transcriptional factors (e.g. Snail) that confer site-specificity