Fig. 2

Dopamine and DRD2 levels significantly increased after chronic stress. A-D Heatmap of targeted metabolomic alterations in plasma (A) and glioma tissues (B) after chronic stress and the quantification results (C&D). E&F Levels of two stress-related neurotransmitters (DA and NE) in plasma (E) and glioma tissues (F) were measured by ELISA. G&H Immunoblotting (G) and immunofluorescence staining (H) of TH in glioma tissues with or without chronic stress. Scale bar: 50 μm. I Clustergram of RNA-seq data from tumor tissues of mice. J KEGG analysis of DEGs of RNA-seq data. K Five key genes in dopamine metabolic pathway were significantly upregulated after chronic stress. L The mRNA levels of DRD1 and DRD2 in glioma xenografts. M&N The protein levels of DRD1-5 in xenografts (M) and the quantified results (N). Representative WB of three independent experiment is shown. TH: tyrosine hydroxylase. *P < 0.05, **P < 0.01, ***P < 0.001