Fig. 3

DRD2 is required for glioma progression induced by chronic stress. A-D Cell proliferation was measured using CCK-8 (A&B) and EdU assay (C&D) in glioma cells over-expressing or knocking-down DRD2.Scale bar: 100 µm. E–H. Representative images and quantitative results of CCK-8 (E&F) and EdU assay (G&H) of glioma cells knocking-down DRD2 with or without NE (20 μM) treatment. I Diagram of in vivo experimental schedule utilizing GBM#2 glioma cells. J&K Time-lapse bioluminescence imaging of mice (J) and the photon flux was analyzed (K). L Kaplan–Meier curve showed the overall survival time of glioma-bearing mice of each group. NE: norepinephrine. *P < 0.05, **P < 0.01, ***P < 0.001