Fig. 4

Chronic stress activates DRD2/ERK/β-catenin axis and Dopamine/ERK/TH regulatory loop. A GSEA was performed using TCGA dataset. B Lysates of glioma tissues after chronic stress were examined by immunoblotting. C Immunofluorescence staining of DRD2 and β-catenin in glioma tissues with or without chronic stress. Scale bar: 50 μm. D Lysates of DRD2 down-regulation or over-expression glioma cells were examined by immunoblotting. E Immunoblotting analysis of DRD2 down-regulated U87 and U251 glioma cells treated with NE. F FpClass analysis predicted that ERK was the protein simultaneously interacting with β-catenin, GSK3β and TH. G Lysates of glioma tissues with or without chronic stress were examined by immunoblotting. H Lysates of DRD2 knockdown U87 and U251 glioma cells treated with both NE and U0126 simultaneously were examined by immunoblotting. WB experiments were independently replicated three times. Act-β-catenin: Active-β-catenin