Fig. 6

Combination treatment of PIMO and TMZ induces a synergistic anti-glioma effect both in vitro and in vivo. A-D Relative proliferation of U251 and U87 cells treated with PIMO (5 μM or 10 μM) and indicated doses of TMZ were measured by CCK-8 assay. E Diagram of in vivo experimental schedule. F&G Representative bioluminescence images of intracranial xenografts using GBM#2 glioma cells with different drug administration on the indicated days (F) and quantitative analysis of the fluorescence index (G). H Kaplan–Meier analysis of the median survival time of nude mice. I Schematic model of how chronic stress promotes glioma growth. PIMO: pimozide. TMZ: Temozolomide. *P < 0.05, **P < 0.01, ***P < 0.001