Fig. 1

Intratumoral administration of TLR9 agonist in osteosarcoma tumors shows therapeutic efficacy on both treated and distant lesions. A. Graphs show mean volume of mOS69 tumors SD101-treated (left panel) and untreated (right panel). Five animals per group were used. A representative experiment is shown (out of 4 experiments performed). Multiple unpaired t tests, one per time points, were used for statistical analysis. B. Representive histological analysis of tumor lesions in Hematoxylin & eosin. C. Representative images of Masson’s Trichrome staining showing a significant increase in collagen (blue staining). D. Representative images of osteocalcin immunohistochemistry, suggestive of increased tumor cell differentiation and matrix deposition