Fig. 2

Intratumoral administration of TLR9 agonist reprograms the tumor microenvironment, decreasing the number of M2 polarized macrophages and increasing dendritic cell infiltration. A. Multiparametric flow cytometry analysis of tumor infiltrating myeloid cells in both treated and untreated lesions from SD101- or PBS-treated mice. Data are expressed as percentage of specific cell subsets (shown on the Y axis). Data from single mice are shown (4–5 mice per group; a representative experiment is shown). Mann–Whitney test was used for statistical analysis. B. Representative immunohistochemistry staining of CD206 on tumor lesions