Fig. 5

Intratumoral administration of TLR9 agonist induces the expansion of specific CD8 T cell clones in both treated and distant lesions. A. Graphs show mean mOS69 tumor volume for SD101-treated (left panel) and untreated (right panel) lesion. Five animals per group were used. B-E. TCR sequencing analysis has been performed on three control samples (both PBS-treated and untreated) and 3 SD101-treated samples (two treated with 25 μg and one with 50 μg, both treated and untreated lesions). Data were analyzed using the ImmunoSEQ Analyzer software. B. Graphs showing total templates (upper panel), max frequency (middle panel) and sample Simpson clonality (lower panel) in each group (control-treated, control-untreated, SD101-treated, SD101-untreated). C. Graph showing the productive frequency of each TCR clone in each samples. Each color represents a specific TCR clone. D. Venn diagrams showing for each mouse the clones shared between treated and untreated lesion. E. Overlap analysis by Morisita index showing overlapping clones between samples from the same mouse or shared between samples from different mice in SD101-treated mice (left panel) and control-treated mice (right panel)