Fig. 2

METTL16 promotes CRC progression in vitro and in vivo. A CCK8 assay was performed to detect the proliferation of SW620 and HCT15 cells with METTL16 knockdown (shM16). B CCK8 assay was performed to detect the proliferation of SW620 and HCT116 cells with METTL16 overexpression. C-D Colony formation assays were performed to detect the proliferation of SW620 and HCT15 cells with METTL16 knockdown (C) or SW620 and HCT116 cells with METTL16 overexpression (D). E-F Transwell assays were performed to detect the migrative and invasive capacity of SW620 cells with METTL16 knockdown (E) or overexpression (F). G, J Xenografts derived from SW620-shM16 or HCT116-OEM16 cells and relative controls (n = 10). H, I, K, L Tumor volume was recorded at indicated time to establish a growth curve (H, K) and tumors weight (I, L) were measured after mice sacrificed. M, N Hematoxylin-eosin (HE) staining and METTL16 and Ki67 IHC staining of xenografts tissues. ***P < 0.001, ****P < 0.0001