Fig. 6

SOGA1 regulates PDK4 expression via inhibiting AMPK activation. A-B The PDK4 protein (A) and mRNA (B) expression in SW620 cells treated with different concentration of AMPK activator A769662 were measured by western blotting and qRT-PCR, respectively. C-D The PDK4 protein (C) and mRNA (D) expression in SW620 cells with or without AMPKα1, β1, γ1 knockdown (siα1, siβ1, siγ1) were measured by western blotting and qRT-PCR, respectively. E-F AMPKα1, β1, γ1 mRNA (E) and protein (F) expression in SW620 cells with or without SOGA1 knockdown were measured by qRT-PCR and western blotting, respectively. G The protein stability of pAMPK, AMPKα1, β1, γ1 were detected in SOGA1-konckdown and control cells via western blotting at the indicated time after CHX (100 µg/ml) treatment. H The capacity of SOGA1 binding to pAMPK, AMPKα1, β1, γ1 were detected by Co-IP. I The ubiquitylation of AMPKα1, β1, γ1, pAMPK were detected in SOGA1-konckdown and control cells by Co-IP. J The PDK4 protein expressions in SOGA1 or METTL16 deficient SW620 cell transfected with siRNAs of AMPKα1, β1, γ1 respectively were detected by western blotting. K A working model of SOGA1 regulating PDK4 expression. **P < 0.01, ***P < 0.001, ****P < 0.0001