Fig.2

Cellular populations of HNSCC correlated with metabolic properties and survival. A Diffusion map of cells from all sc-RNA samples, colored by cell-type annotation. B Diffusion map of malignant cells colored by cell proportion annotation, including 9 clusters. C KEGG pathway enrichment analysis of DEGs between primary- and metastatic-specific populations. D GSEA analysis of “META_ACTIVATE” gene signatures between primary- and metastatic-specific populations, which were at the right and left side respectively. E AUC score of all cells from HNSCC samples. The threshold was marked by red bar and the cells colored by darked blue exceeded the threshold value. F Diffusion map based on the AUC score with cells exceeded the threshold value marked. G Relative abundance of different cell populations by gene expression deconvolution in each patient from METArisk-high and METArisk-low groups. Each bar represents an individual patient, and the distribution of colors throughout each bar represents the distribution of cells. H Kaplan–Meier analysis for survival probability of the two METArisk groups