Fig.3

Identification of PYGL as a significant metabolic biomarker. A Venn diagram among METArisk-related DEGs, tumor-related DEGs and MRGs. B SVM-RFE used to select features having best discriminative capability for patient’s prognosis of HNSCC. C Multivariate Cox regression analyses discovered DEGs that can serve as independent predictors of clinical outcomes. D Kaplan–Meier survival curves of the four genes selected above. E Boxplot depicted the expression level of the four genes in METArisk-high and METArisk-low groups. F Representative IHC images of the four genes from the HPA database. G qRT-PCR analysis of the four genes. Relative mRNA expression level of tumor tissue compared with paracancerous tissure were exhibited in bar chart (n = 3). H The summary image of the four genes with research findings along with the biological functions