Fig.4

HNSCC malignant behaviors suppressed after PYGL knock-down. A and B qRT-PCR (A) and WB (B) analysis of the transfaction efficiency (n = 3 for independent experiments). C CCK-8 assay were used to detect cell proliferation capacities (n = 3). D and E Transwell assay were performed to examine cell potential of migration (D) and invasion (E). F Immunofluorescence staining showed the DNA damage (stained by γ-H2AX, red) in cells. Nuclei were counterstained with DAPI (blue) (n = 3). G Cell apoptosis was measured by flow cytometry assay. Cell was stained by AnnexinV and SSA-C and apoptotic cell was emphasized (n = 4)