Fig. 1

A) Representative images of immunohistochemical analysis of F4/80, CD206 and Bcl-xL expression in melanoma control (Mneo) and Bcl-xL overexpressing (Mxl90) tumour-bearing mice, performed 30 days after subcutaneous cell injection (20X magnification), and B) relative quantification of peritumoral (PT) and intratumoral (IT) macrophage infiltration by F4/80 and CD206 staining. The results are reported as mean score: score 0, no detectable infiltrate; score 1, low infiltrate; score 2, moderate infiltrate; score 3, high or very high infiltrate. p-values were calculated between control and Bcl-xL overexpressing tumours. C) Representative images of Mneo and Mxl90 cells 5 days after injection in the yolk sac of 2 days post fertilization Tg (mpeg1: Cherry/tnfa: GFP) zebrafish larvae, and D) relative quantification of M1 (mCherry+/eGFP+) and M2 (mCherry+/eGFP-) macrophages recruitment in the tail.  B,D) Each dot (•) indicates an experimental point, *p < 0.05, ***p < 0.01; ns, no significant differences