Fig. 5

A) qRT-PCR analysis and B) ELISA of IL-1β, IL-8 and CCL5 expression in control (Mneo) and Bcl-xL overexpressing melanoma cells transfected with IKBSR (Mxl90 IKBSR) or control (Mxl90) vectors. Fold induction relative to Mneo cells and the average ± SEM of three experiments is reported. The average ± SEM of three independent experiments is reported. C) Representative images (left panels) and relative quantification (right panel) of M-DM migration in response to CM from Mneo (CM Mneo), Mxl90 (CM Mxl90) and Mxl90 IKBSR (CM Mxl90 IKBSR) cells. The quantification was performed by counting the number of migrated cells in at least 10 fields for each condition. p-values were calculated between the percentage of migrated cells exposed to CM derived from Bcl-xL overexpressing clone transfected with CMV or IKBSR. D) Representative images of Mneo, Mxl90 and Mxl90 IKBSR cells (red arrows) 5 days after injection in the yolk sac of 2 days post fertilization Tg (mpeg1: EGFP) zebrafish larvae, and relative quantification of E) recruited macrophages (green arrows) and F) invasion score of melanoma cells invading the tail. *p < 0.05, **p < 0.01, ***p < 0.001