Fig. 5

CD271 modulation improves PDT or chemotherapy efficiency a Schematic representation of photodynamic therapy (PDT) on SCC spheroids. b Cell viability evaluated at 24 h in mock vs CD271-overexpressing SCC13-derived spheroids treated with PDT for 15 min, 1 h or 3 h (n = 3). c Left panel: Spheroids total area, corresponding to 3 h PDT treatment, evaluated at 24 h. Right panel: representative brightfield images and ImageJ mask of mock and CD271-overexpressing spheroids d Representative images for PI staining (red)/brightfield of mock vs CD271 spheroids after treatment with 5FU (1, 10 and 50 μg/ml) or DMSO (control) at 48 h. Scale bar = 100 μm e Histogram representing PI staining/total spheroid area ratio (for each condition, n = 3). Results are shown as mean ± SD. Statistical analysis was performed using the two-way ANOVA and multiparametric t-test. *0.01 < p < 0.05, ***0.0001 < p < 0.001, ****p < 0.0001