Fig. 2

Analysis of residual tumor burden following chemotherapy. Mice on either control LFD (n = 11) or HFD (n = 10) were injected with 1 × 10⁷ RFP-tagged ID8-Trp53^−/− cells. After three weeks, mice were treated with 9 cycles of weight-adjusted paclitaxel (6 mg/kg) and carboplatin (15 mg/kg). One week following the last chemotherapy treatment, mice were sacrificed, the peritoneal cavity exposed with a midline incision, and RFP signal was imaged in situ using an Ivis Lumina to determine (A, B) total abdominal tumor burden. C, D Abdominal organs were then removed and imaged ex vivo to evaluate organ-specific tumor burden. Graphs show mean and standard error of the mean. Pairwise statistical analyses were conducted using Student’s t-test (Sigmaplot). n.s. = not significant (p > 0.05)