Fig. 3

Evaluation of tumor-associated macrophage (TAM) staining in human ovarian tumors from patients with normal vs high body mass index (BMI). Tissues were stained using MultiOmyx technology as described. Regions of interest (12–28) were identified by a pathologist. M1 and M2 TAMS were quantified by applying the proprietary deep-learning based cell classification platform NeoLYTX to multiplexed images. A, B Representative color overlay images of tumors from patients with normal body mass index (BMI) and high BMI. Arrows indicate examples of M1 TAMs (yellow) and M2 TAMs (magenta). C Quantification of TAM staining data. Pairwise statistical analyses were conducted using Student’s t-test (Sigmaplot). n.s. = not significant (p > 0.05). Box and whisker plots show lower quartile, median, and upper quartile (box) and minimum/maximum values (whiskers)