Fig. 4

Evaluation of TAM staining in murine tumors from low fat diet (LFD) and high fat diet (HFD) mice. A, B Sections were stained for M1 TAMs using anti-iNOS antibodies (1:1000 dilution) followed by peroxidase-conjugated secondary antibody and peroxidase detection. C, D Sections were stained for M2 TAMS using anti-CD206 antibodies (1:6400 dilution) followed by peroxidase-conjugated secondary antibody and peroxidase detection. E–G Stained sections were quantified using an Aperio Scanscope and analyzed using ePathology ImageScope software percent positive macro algorithm to quantify the number of DAB chromogen positive (brown) cells. Two slides were scanned per tumor and a minimum of 10 regions of interest per slide were evaluated. Statistical analysis was completed using Student’s t-test (Sigmaplot). Box and whisker plots show lower quartile, median, and upper quartile (box) and minimum/maximum values (whiskers)