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Table 3 Adipokine array analysis of ascites from mice on low fat diet (LFD) or high fat diet (HFD). A Proteome Profiler Mouse Adipokine Array was used to profile the expression of adipokines in ascites (n = 3 individuals per cohort, duplicate samples). Samples were normalized for protein content and analyzed according to manufacturer’s specifications. Array results were detected with an Image Quant LAS 4000 and quantified using ImageQuant™ TL software. Data are reported using the arbitrary unit of “volume” that incorporates the signal area and signal intensity

From: Host obesity alters the ovarian tumor immune microenvironment and impacts response to standard of care chemotherapy

Adipokine

Low fat diet ascites

(volume units × 10–5)

High fat diet ascites

(volume units × 10–5)

P value

Adiponectin

4.98 +/- 0.29

3.35 +/- 0.14

0.001

FGF21

0.06 +/- 0.01

0.75 +/- 0.14

0.002

IGFBP-2

6.32 +/- 0.94

4.51 +/- 0.85

0.006

IGFBP-3

3.36 +/- 0.08

2.46 +/- 0.22

0.004

IGFBP-5

0.24 +/- 0.08

0.06 +/- 0.01

0.037

IGFBP-6

3.44 +/- 0.05

2.41 +/- 0.83

0.002

  1. Abbreviations: FGF21 Fibroblast growth factor 21, IGFBP Insulin-like growth factor binding protein