Fig. 5

TRAF2 promotes cell proliferation, migration, and invasion in ccRCC cells. (A, B) Stable knockdown of TRAF2 by shRNAs effectively decreased its both mRNA and protein expression level in OSRC2 and CAKI2 cells. (C) Representative images from the colony formation assay (left panel) and colony number analysis (right panel). All experiments were performed in triplicate and data were presented as the mean ± SD. (D) Representative 3D soft agar growth pictures (left panel) and quantification of colony number (right panel). (E) Representative cell migration pictures (left panel) and quantification of cell migration (right panel). (F) Representative cell invasion pictures (left panel) and quantification of cell invasion (right panel). Error bars, SEM; **, p < 0.01; ***, p < 0.001, ****, p < 0.0001