Fig. 6

TRAF2 promotes ccRCC progression via regulating macrophage polarization, migration, and angiogenesis. (A) Pearson correlation analysis between TRAF2 expression and the markers of M2 macrophages (CD206 and CD301). (B) RT-qPCR analysis was used to detect the changes of CD14 and CD11b mRNA expression in THP-1 cells treated with PMA (200 nM, 48 h). (C) RT-qPCR analysis was used to detect the markers of M2 macrophages (CD206 and CD301) mRNA expression changes in macrophage-like cells co-cultured with shCtrl or shTRAF2 OSRC2 cells. (D) Western Blot analysis was used to detect VEGFA protein expression changes in macrophage-like cells co-cultured with shCtrl or shTRAF2 ccRCC cells. (E) Representative tube-formation pictures of in vitro angiogenesis (left panel) and quantification of the node branches (right panel). (F) Representative cell migration pictures of macrophages (left panel) and quantification of cell migration (right panel). Error bars, SEM; **, p < 0.01; ***, p < 0.001, ****, p < 0.0001