Fig. 7

TRAF2 induces macrophage polarization in an autophagy-dependent pathway. (A) GSEA analysis was performed in TCGA-KIRC cohort to reveal the association between TRAF2 and the activation of autophagy pathway. (B, C) Transmission electron microscopy (TEM) demonstrating autophagosomes in shCtrl and TRAF2 depleted OSRC2 and CAKI2 cells. Red arrows indicate autophagosomes. (D) Quantification of autophagosomes in cells. (E) RT-qPCR analysis was used to detect CD206 and CD301 mRNA expression in macrophage-like cells with rapamycin treatment (100 nM, 48 h). (F) Representative tube-formation pictures of in vitro angiogenesis. (G) Quantification of the node branches. (H) Western Blot analysis of the effect of autophagy activator on the expression of VEGFA protein. (I) Representative cell migration pictures of macrophages (left panel) and quantification of cell migration (right panel). Error bars, SEM; **, p < 0.01; ***, p < 0.001