Fig. 8

TRAF2 is critical for ccRCC tumorigenesis and metastasis in vivo. (A) Images of the orthotopic transplantation mouse model (shCtrl and shTRAF2#1). (B) Quantification of the orthotopic tumor photon flux. (C) Image of orthotopic transplanted tumors from shCtrl and shTRAF2#1 groups. (D, E) Tumors were removed 8 weeks after orthotopic transplantation, followed by volume calculation and weight measurement. (F) Representative IHC staining images of TRAF2 and Ki67. (G, H) Quantification of TRAF2 expression and Ki67 positive rate. (I, J) Representative images of the orthotopic transplantation lung metastasis mouse model model (shCtrl and shTRAF2#1) and the analysis of the lung metastasis photon flux. (K) Representative images of mouse lung gross specimens, hematoxylin and eosin (H&E) staining, and IHC staining for E/N-cadherin. (L) The survival of mice orthotopically transplanted with OSRC2-luciferase cells expressing shCtrl or shTRAF2#1 was documented (n = 10 mice per group, p = 0.008). Error bars, SEM; *, p < 0.05; **, p < 0.01; ***, p < 0.001, ****, p < 0.0001