Fig. 2

The rWTC-MBTA vaccine prevents tumor metastasis in breast and melanoma metastasis animal models. a, Treatment schedule for immunotherapy. b&e, Representative photographs of lung tissue from animal models of 4T1 or B16 metastasis. The black arrows indicate tumor metastasis foci (n = 5/group). c&f, Representative H&E staining of lung tissues collected on day 45 from (c) 4T1 or (f) B16 metastatic animal models (2× scale bar = 600 μm; 20 × scale bar = 60 μm) (n = 5/group). d&g, Quantification of metastatic foci in the lung mice using (d) 4T1 or (g) B16 metastasis model (n = 5/group). Ordinary one-way ANOVA with multiple comparisons was used to calculate statistical significance. *P < 0.05, **P < 0.01, ***P < 0.001,**** P < 0.0001.All data were represented as mean ± SD. P-values shown are for each treated group comparison