Fig. 3

rWTC-MBTA vaccine inhibits postoperative tumor metastasis in 4T1 breast cancer animal model. a, Treatment schedule for immunotherapy in breast animal model with surgical tumor removal. b, Represantative H&E staining of lung tissue collected each at various time points. Scale bar = 100 μm (n = 3/group). c, IHC for CD4 and CD8 staining of lung tissue collected at different time points (n = 3/group). Scale bar = 100 μm. d, Overall survival curve of mice after control and vaccine treatments (n = 7/group). Survival rate was analyzed using the Mantel-Cox (log-rank) test. e, Treatment schedule for rechallenge with subcutaneous tumor cells in mice that survived the initial challenge. f, Mean and individual tumor growth curves for mice receiving different treatments (n = 4 for control, n = 2 for re-challenged survival mice). The mean tumor volumes were analyzed using Kruskal-Walllis test with multiple comparisons. All data were represented as mean ± SD. P-values are shown for control versus rWTC-MBTA vaccine