Fig. 4

The rWTC-MBTA vaccine against autologous tumor metastasis and growth but not allogeneic tumor metastasis and growth. a, Treatment schedule for tumor growth and metastasis inhibition by the rWTC-MBTA vaccine. b, Mean and individual tumor growth curves for mice receiving different treatments (n = 5/group). The mean tumor volumes were analyzed using one-way ANOVA. c, Representative H&E staining of lung tissue collected at day 45 from the primary mammary animal model (2× scale bar = 600 μm; 20 × scale bar = 60 μm) (n = 3). d, Quantification of lung metastasis nodes in each group (n = 5/group). Ordinary one-way ANOVA with multiple comparisons was used to assess statistical significance. All data were represented as mean ± SD. P-values were shown for each treated group compared to rWTC-MBTA vaccine or r4T1. e, Cross-vaccination experiment design. f, Mean and individual tumor growth curves of the tumors receiving different treatments (n = 3/group). The data were presented as mean ± SD. g, Representative H&E staining of lung tissue collected on day 45 (2× scale bar = 600 μm; 20 × scale bar = 60 μm) (n = 3/group). h, Quantification of lung metastasis nodes in each group (n = 3/group). Ordinary one-way ANOVA with multiple comparisons was used to assess statistical significance. All data are represented as mean ± SD. P-values shown are for each treated group comparison. *P < 0.05, **P < 0.01, ***P < 0.001,**** P < 0.0001