Fig. 2

B.adolescentis recruited macrophages to suppress colorectal tumorigenesis. A Schematic representation of single cell preparation and scRNA-seq. B Myeloid cells were divided into 5 groups according to the top expression markers. C The percentage of 5 groups in B.adolescentis and vehicle (PBS) groups. D-E Flow cytometry representation and percentage of macrophages in colorectal lamina propria. F-G The number of F4/80⁺ cells was examined by immunofluorescence in the colorectal tumor of AOM/DSS mice treated with B.adolescentis, E.coli and vehicle (PBS); scale bars, 20 μm. H-J HCT116 cells were injected into BALB/c nude mice combined with THP-1 cells pretreated with B.adolescentis, E. coli or vehicle (PBS) for 24 h (n = 5 per group). Tumor volume and weight were recorded after 6 days. The independent experiment was repeated three times. Data are shown as mean ± SD. ** P < 0.01, *** P < 0.001, **** P < 0.0001; ANOVA test (E, G, I, J)