Fig. 3

B.adolescentis facilitated the infiltration of Decorin⁺ macrophages to suppress CRC. A The heatmap of RNA-seq of macrophages treated with B.adolescentis or vehicle (PBS). B The volcano plot showed differentially expressed genes in scRNA-seq of macrophages treated with B.adolescentis or vehicle (PBS). C 3852 differentially expressed genes in RNA-seq and 1036 differentially expressed genes of macrophages in scRNA-seq were subjected to Venn diagram analysis (|log₂fold change|> 0.5, p.adj < 0.05), and 11 shared differentially expressed genes were acquired. D The levels of 11 differentially expressed genes in BMDMs treated with B.adolescentis were determined by qRT-PCR. E, F The number of DCN⁺F4/80⁺ cells was examined by immunofluorescence in the colorectal tumor of AOM/DSS mice treated with B.adolescentis, E. coli or vehicle (PBS); The yellow arrows indicate the positively stained cells. scale bars, 20 μm. G BMDMs or human macrophage THP-1 cells were incubated with B.adolescentis or vehicle (PBS) for 24 h. Protein level of DCN was measured by Western blot. H Raw264.7 cells was incubated with B.adolescentis or vehicle (PBS) for 24 h. Protein level of DCN was measured by Western blot. I The protein level of DCN in Raw264.7 Dcn-knockdown cells or Raw264.7 control cells. J-L Raw264.7 Dcn-knockdown cells or control cells were pretreated with B.adolescentis or vehicle (PBS), and combined with CT26 were injected into BALB/c nude mice. Tumor volume was recorded after 6 days. The independent experiment was repeated three times. Data are shown as mean ± SD. ns: No statistical difference, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; Student t test (D), ANOVA test (F, K, L)