Fig. 6

B.adolescentis abundance correlated with TLR2 and DCN⁺ macrophages in patients with CRC. A Representative images of immunofluorescence assay for CD68 in tumor tissue with low or high B.adolescentis abundance from cohort; scale bars, 50 μm. B Representative images of immunofluorescence staining of DCN⁺ macrophages (CD68⁺DCN⁺ dual positive) in tumor tissue of CRC; scale bars, 50 μm. The yellow and white arrows indicate the positively stained cells. C qRT-PCR analysis of DCN and TLR2 mRNA expression in tumor or para-tumor tissue of CRC. D The correlation of B.adolescentis with DCN or TLR2 was analyzed in tumor tissue of CRC. E The correlation of DCN and TLR2 was analyzed in tumor tissue of CRC. F Transcript levels of DCN and TLR2 in colorectal cancer and normal tissue from TCGA database. G The correlation of DCN and TLR2 was analyzed in TCGA database. The independent experiment was repeated three times. Data are shown as mean ± SD. * P < 0.05, **** P < 0.0001; Mann–Whitney test (A, C, F), Linear Regression (D, E, G)