Fig.2

Comprehensive transcriptome analysis by RNA-seq of wild type and RAGE-overexpressing MCF7 cells. A Volcano plot evidencing the differentially expressed genes (DEGs) in RAGE-overexpressing (MCF7/RAGE) respect to wild type (MCF7/wt) MCF7 cells, as ascertained by RNA-seq analysis. p-value < 0.05 was considered as a significant threshold; significantly down-regulated genes (log2FC ≤ 0.5 and p < 0.05) are shown in blue (n = 1340), significantly up-regulated genes (log2FC ≥ 0.5 and p < 0.05) are shown in red (n = 725), non-significant genes are shown in grey (p > 0.05). Side box shows enrichment scores for gene ontology (GO) terms of the up-regulated DEGs in MCF7/RAGE versus MCF7/wt cells, involved in biological process (BP), molecular function (MF), cellular component (CC), according to p < 0.05 and log2FC > 0.5. B Interrelation analysis of “cell–cell adhesion”, “chemotaxis”, “biological adhesion” and “cell adhesion” BP and relative genes. Survival analysis showing the association of high expression of the genes belonging to the “chemotaxis” (C) and “biological adhesion” (D) BP with a worse disease specific survival (DSS) in ER-positive BC patients of the TCGA cohort