Fig.4

Growth and dissemination of RAGE-overexpressing BC cells. A-B Colony formation in wild type (MCF7/wt and T47D/wt) and RAGE-overexpressing (MCF7/RAGE and T47D/RAGE) MCF7 and T47D cells. After 10 days of incubation cell colonies were stained and pictures were captured by a digital camera. Colonies were counted using the program WCIF ImageJ for Windows. Each data point is the mean ± SD of three independent experiments performed in triplicate. C,F DiI-labeled wild type (MCF7/wt and T47D/wt) and RAGE-overexpressing (MCF7/RAGE and T47D/RAGE) cells were injected into the yolk sac of 48 hpf larvae, and tumor cell growth and dissemination were detected using fluorescent microscopy at day 3 post-injection. Yellow arrows indicate primary tumors. White arrows indicate disseminated tumor foci. D,G Quantification of DiI-labeled tumor volume (n = 6/group). The mean value of the tumor size in wild type zebrafish xenografts was settled as 100%. E,H Quantification of numbers of disseminated tumor foci (n = 6/group). Data are represented as mean ± SD. (*) indicates p < 0.05