Fig.6

The EphA3 signaling mediates the motile phenotype of RAGE-overexpressing BC cells and is associated with a worse prognosis in BC patients. Transwell migration (A-B) and invasion (C-D) assays in MCF7/RAGE and T47D/RAGE cells in the presence or absence of EphA3 silencing. Efficacy of EphA3 silencing in MCF7/RAGE (E) and T47D/RAGE (F) cells. Side panel shows densitometric analysis of the blots normalized to β-actin. Transwell migration (G-H) and invasion (I-J) assays in MCF7/RAGE and T47D/RAGE cells in the presence or absence of 10 μM EphA3 inhibitor AWL-II-38.3. Cells were counted in at least 5 random fields in three independent experiments performed in triplicate, as quantified in the side panels. Scale bar: 200 μm. Values represent the mean ± SD of three independent experiments performed in triplicate. (*) indicates p < 0.05