Fig. 3

ISG15 and ISGylation were significantly enriched in cancer stem cells. A The Pearson correlation analysis of ISG15 and stemness. B The mRNA level of ubiquitin-like proteins in con or sphere groups. Immunofluorescence staining of ISG15 and stemness marker CD133 in (C) control or sphere groups and (D) clinical ATC patient samples. E Flow cytometry analysis of ISG15 expression in CD133 + 8505 C or KHM5M cells. Western blot to analyze ISG15 and ISGylation expression in (F) control or sphere groups and G CD133^± ATC cells