Fig. 4

The silence of ISG15 inhibited CSCs characteristics. A The knockdown efficiency of ISG15 and ISGylation analyzed by western blot. B The cell viability, (C) clone formation, (D) sphere formation and (E) mRNA level of OCT4, NANOG and CD133 of 8505 C or KHM5M cells after ISG15 silence. F The cell viability of 8505 C or KHM5M cells after ISG15 silence combined with doxorubicin (0, 0.5, 1, 2, 4 and 8 µM) for 48 h. G The mRNA level of ABCB1 and ABCC1 of 8505 C or KHM5M cells after ISG15 silence