Fig. 5

The overexpression of ISGylation promoted CSCs characteristics. A The overexpression efficiency of ISGylation-WT/AA analyzed by western blot. B The cell viability, (C) sphere formation and (D) mRNA level of OCT4, NANOG and CD133 of 8505 C or KHM5M cells after ISGylation-WT/AA overexpression. E The cell viability of 8505 C or KHM5M cells after ISGylation-WT/AA overexpression combined with doxorubicin for 48 h. F The mRNA level of ABCB1 and ABCC1 of 8505 C or KHM5M cells after ISGylation-WT/AA overexpression