Fig. 6

KPNA2 was the substrate for ISG15 to modulate CSCs characteristics. A The Venn diagram for screening potential substrates. B The expression of KPNA2 in NT or ATC tissues. C Co-immunoprecipitation to detect the interaction of endogenous ISG15 with KPNA2 in ATC cell lines. D Co-immunoprecipitation to detect the interaction of exogenous ISGylation-WT/AA with KPNA2. E The Pearson correlation analysis of KPNA2 and stemness. Western blot to analyze KPNA2 expression in (F) control or sphere groups and (G) CD133^± ATC cells