Fig. 7

Deletion of ISGylation promoted the ubiquitination and degradation of KPNA2. A The pearson correlation analysis of KPNA2 and ISG15. B Western blot to analyze KPNA2 expression after ISG15 silence in ATC cells. C Western blot to analyze KPNA2 expression after ISG15 silence combined with 20 ug/ml Cycloheximide (CHX) at the 0, 6, 9 and 12 h. D Western blot to analyze KPNA2 expression after ISG15 silence combined with 10 mM MG132 or not. E Co-immunoprecipitation to detect the ubiquitination of KPNA2 after ISG15 silence combined with 10 mM MG132