Fig. 8
KPNA2 mediated CSCs characteristics regulated by ISG15 and ISGylation. A The knockdown efficiency of KPNA2 analyzed by western blot. B The sphere formation and (C) mRNA level of OCT4, NANOG and CD133 of ATC cells after KPNA2 silence. D Immunofluorescence staining of c-MYC after KPNA2 silence in ATC cells. E The sphere formation and (F) mRNA level of OCT4, NANOG and CD133 after ISGylation-WT overexpression combined with KPNA2 silence or not