Fig. 9

Knockdown of ISG15 inhibited tumorigenesis and metastasis of ATC in vivo. Subcutaneous tumor xenografts in nude mice established by 8505 C-NC or ISG15-KD. A The growth curve of the tumor. B mice body weight. C Tumor weight. D Flow cytometry analysis of CD133 expression in tumor tissues. E The expression of ISG15, ISGylation, KPNA2 and stemness markers analyzed by PCR. F Schematic diagram of the zebrafish models. G The tumorigenesis of zebrafish in control and ISG15-KD groups. H The metastasis of zebrafish in control and ISG15-KD groups. Red fluorescence represents labeled ATC tumor cells