Fig. 2

Targeting TRF2 by LNPs-miR-182-3p in triple-negative breast cancer. A Targeting of 3'UTR-TRF2 by selected miRNAs was tested by high-throughput luciferase screening. B Western blot of TRF2 expression in tumor cells transfected with miR-182-3p or miR-Control is shown. C Representative images show the telomeric and pericentromeric DNA damage induced by miR-182-3p in MDA-MB-231 cells. D Cell confluence or apoptosis activation in MDA-MB-436 cells, transfected with the indicated miRNAs, were monitored by live-cell imaging (Incucyte) or analysed by flow cytometry (FACS), respectively. E Scheduling of treatment (top/right panel); Analysis of tumor inhibition after treatment with LNPs-miR-182-3p or its relative controls (bottom/right panel); TRF2 and γH2AX expression in tumor tissues was analyzed by immunohistochemistry (IHC) (middle panel); Representative images show mice with artificial brain tumor generated by intracranial injection of MDA-MB-231. Mice were treated with LNPs-miR-182-3p or its control (left panel)