Fig. 4

Validation of the in silico screening with CRC PDOs. a Representative micrographs of two PDO cultures treated with 0.05% DMSO (ctrl)- or pentoxifylline(p) and subsequently with saline-(ctrl) or 5-FU. Micrographs taken at 72 h after treatment started. Scale bar: 200 µm. b Graphs showing the RS score for the PDOs treated with ctrl (DMSO 0.05%) or with the compound A (dexketoprofen), B (perphenazine), C (desloratadine) or D (pentoxifylline) and challenged 6.5 h later with ctrl (saline) or 5FU. The RS score was obtained according to the following formula: number of formed organoids x average max diameter at time 0 day / number of organoids x average max diameter after 72 h, as described in the methods section. Statistics: * p < 0.05; ** p < 0.01; no asterisk: not significant