Fig. 7

Treatment with pentoxifylline attenuated the release of IL-6 after 5-FU treatment. a Upper panel. Graph showing the levels of IL-6 quantified in the conditioned medium of the ctrl and 5-FU-treated PDOs. Results are expressed in nanograms per milliliter of IL-6, adjusted for 10^6 PDO-derived cells, after 48 h of medium conditioning and representative of three independent experiments. b Left panel: Representative immunocytochemistry of PDO#4-derived cells treated with ctrl (a), pentoxifylline (b), 5-FU (c) or pentoxifylline + 5-FU (d), cytospun and stained, after fixation, with an IL-6-antibody. Size bar, 100 µm. Right panel: Box plots showing the percentage of IL-6 positive cells (upper panels) and the mean intensity of the IL-6 signal (lower panel) in the PDO#1 and PDO#4 treated and stained as indicated in the left panel